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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Description:
5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice- to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
- to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
- to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C42H54N5O21P3 (free acid)
Molecular Weight: 1057.83 g/mol (free acid)
Exact Mass: 1057.25 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in 100 mM Tris-HCl
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA/cDNA by- PCR with Taq polymerase in-house data
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产品组分
内容
型号
规格 储存温度
5-DBCO-PEG4-dUTP
CLK048-01 50ul(10mM)
-20°C 5-DBCO-PEG4-dUTP
CLK048-02 250(10mM)
-20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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High-yield ligation-free assembly of DNA constructs with nucleosome positioning sequence repeats for single-molecule manipulation assays.
Yi-Yun Lin, Tine Brouns, ..., Jan Lipfert
The Journal of Biological Chemistry | 2023 Jul 31 | 37257819
Supplementary Click-Particle Display for Base-Modified Aptamer Discovery
Chelsea K. L. Gordon, Diana Wu, ..., Hyongsok Tom Soh
ACS chemical biology | 2019 Sep 18 | 31532184
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