叠氮-PEG4-氨烯丙基-dUTP
叠氮-PEG4-氨烯丙基-dUTP，Azide-PEG4-aminoallyl-dUTP，5-(15-Azido-4,7,10,13-tetraoxa-pentadecanoyl-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt，5-(15-叠氮基-4,7,10,13-四氧杂-十五烷酰基-氨基烯丙基)-2'-脱氧尿苷-5'-三磷酸三乙铵盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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叠氮-PEG4-氨烯丙基-dUTP
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目录号
NU1705
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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Description:
Azide-PEG4-aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Azide-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-catalyzed or Cu(I)-free click chemistry that offers the choice- to introduce a Biotin group (via Alkynes of Biotin or DBCO-functionalized Biotin, respectively) for subsequent purification tasks
- to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectively) for subsequent microscopic imaging
- to crosslink the DNA to Azide- or DBCO-functionalized biomolecules e.g.proteins
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C23H39N6O19P3 (free acid)
Molecular Weight: 796.51 g/mol (free acid)
Exact Mass: 796.15 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Concentration: 10 mM - 11 mM
pH: 7.5 ± 0.5
Spectroscopic Properties: λmax 289 nm, ε 7.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA/cDNA by- PCR with Taq polymerase in-house data
- 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[1]
Selected References:
[1] Sorensen et al. (2013) Enzymatic Ligation of Large Biomolecules to DNA. ACS Nano 7 (9):8098.
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产品组分
内容
型号
规格 储存温度
叠氮-PEG4-氨烯丙基-dUTP
NU1705-01 50ul(10mM)
-20°C 叠氮-PEG4-氨烯丙基-dUTP
NU1705-02 250ul(10mM)
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Patent Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
Saurabh Rajendra Nirantar, Sergio Peisajovich, Eric Brustad
us12209266 | 2025 Jan 28
DNA-enhanced CuAAC ligand enables live-cell detection of intracellular biomolecules
Nian Keqing, Liu Yifang, ..., Rouhanifard Sara H.
bioRxiv | 2022 Nov 10
Patent Super-resolution sequencing
Kalim U. Mir
us11066701 | 2021 Jul 20
Supplementary Nucleotidyl transferase assisted DNA labeling with different click chemistries
Marie-Luise Winz, Eva Christina Linder, ..., Andres Jäschke
Nucleic Acids Research | 2015 Sep 30 | 26013812
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