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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C11H15N2O14P3 (free acid)
Molecular Weight: 492.16 g/mol (free acid)
Exact Mass: 491.97 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2]
Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1]
The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice- to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
- to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
- to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins
Presolski et al.[3] and Hong et al.[4] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
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产品组分
内容
型号
规格 储存温度
5-乙炔基-dUTP(5-EdUTP)
CLKT07-01 10ul(100mM)
-20°C 5-乙炔基-dUTP(5-EdUTP)
CLKT07-02 50ul(100mM)
-20°C 5-乙炔基-dUTP(5-EdUTP)
CLKT07-03 100ul(100mM)
-20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Gierlich et al. (2007) Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry. Chem. Eur. J. 13:9486.
[2] Burley et al. (2006) Directed DNA Metallization. J. Am. Chem. Soc. 128 (5):1398.
[3] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[4] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cells
Vojtěch Pavluch, Tomáš Špaček, ..., Petr Ježek
Scientific Reports | 2023 Apr 08 | 37031254
Patent Glycosylated oligonucleotides that mimic the HIV-1 epitope of neutralizing monoclonal antibody 2G12
Isaac J. Krauss, Joel Sebastian Temme
us10780150 | 2020 Sep 22
Quantitation of deoxynucleoside triphosphates by click reactions
Chang-Yu Huang, Miriam Yagüe-Capilla, ..., Zee-Fen Chang
Scientific Reports | 2020 Jan 17 | 31953472
Patent High temperature selection of nucleotide-supported carbohydrate vaccines and resulting glycosylated oligonucleotides
Isaac J. Krauss
us10378017 | 2019 Aug 13
Genetic Mapping and Biochemical Basis of Yellow Feather Pigmentation in Budgerigars
Thomas F. Cooke, Curt R. Fischer, ..., Carlos D. Bustamante
Cell | 2018 May 14 | 28985565
SELMA: Selection with Modified Aptamers.
J Sebastian Temme, Isaac J Krauss
Current protocols in chemical biology | 2016 May 12 | 26344234
Supplementary Nucleotidyl transferase assisted DNA labeling with different click chemistries
Marie-Luise Winz, Eva Christina Linder, ..., Andres Jäschke
Nucleic Acids Research | 2015 Sep 30 | 26013812
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