脱氧三磷酸(dNTP)预混液(25mM)
脱氧三磷酸(dNTP)预混液(25mM)，dNTP Mix - 25 mM Solution，Equimolar Mix of 25 mM dATP, dCTP, dGTP and dTTP，25毫摩尔dATP、dCTP、dGTP和dTTP的等摩尔混合物，2'-Deoxyadenosine-5'-triphosphate, Sodium salt; 2'-Deoxycytidine-5'-triphosphate, Sodium salt; 2'-Deoxyguanosine-5'-triphosphate, Sodium salt; 2'-Deoxythymidine-5'-triphosphate, Sodium salt，2'-脱氧腺苷-5'-三磷酸 钠盐；2'-脱氧胞苷-5'-三磷酸 钠盐；2'-脱氧鸟苷-5'-三磷酸 钠盐；2'-脱氧胸苷-5'-三磷酸 钠盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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脱氧三磷酸(dNTP)预混液(25mM)
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NU1023
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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Description:
dNTP Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP supplied as clear aqueous solution (pH 8.5).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula:
dATP: C10H16N5O12P3 (free acid)
dCTP: C9H16N3O13P3 (free acid)
dGTP: C10H16N5O13P3 (free acid)
dTTP: C10H17N2O14P3 (free acid)Molecular Weight:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dTTP: 482.17 g/mol (free acid)Purity: ≥ 99 % (HPLC)
Form: clear aqueous solution
pH: 8.5 ±0.2 (22 °C)
Applications:
For standard PCR applications a final concentration of 200 μM each dNTP is recommended.Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable -
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脱氧三磷酸(dNTP)预混液(25mM)
NU1023-01 1ml
-20°C 脱氧三磷酸(dNTP)预混液(25mM)
NU1023-02 5ml
-20°C 脱氧三磷酸(dNTP)预混液(25mM)
NU1023-03 10ml
-20°C 脱氧三磷酸(dNTP)预混液(25mM)
NU1023-04 100ml
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.Sex-specific molecular and cellular phenotypes during resolution of neuropathic pain in dorsal root ganglia.
Felicitas Schlott, Beate Hartmannsberger, ..., Robert Blum
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Chemical Science | 2025 Feb 11
Enzymatic bypass of G-quadruplex structures containing oxidative lesions
Peter Podbevšek, Janez Plavec
Nucleic Acids Research | 2025 Jan 13 | 39673512
Variants in the Control Region of Mitochondrial Genome Associated with type 2 Diabetes in a Cohort of Mexican Mestizos.
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Archives of medical research | 2023 Mar 07 | 36792418
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Michal Tamáš, Alena Potocarova, ..., Tomas Mackulak
International Journal of Environmental Res… | 2022 Aug 15 | 35955106
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Journal of the American Chemical Society | 2022 Jun 06 | 35666649
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Supuni Thalalla Gamage, Aldema Sas-Chen, ..., Jordan L. Meier
Nature Protocols | 2022 May 04 | 33772246
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Changtong Wu, Daria Barkova, ..., Dirk Mayer
Analytical and Bioanalytical Chemistry | 2022 Mar 08 | 34783880
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Ilir I Cinoku, Kleio-Maria Verrou, ..., Clio P Mavragani
Journal of autoimmunity | 2022 Feb 21 | 34298409
Novel Nested-Seq Approach for SARS-CoV-2 Real-Time Epidemiology and In-Depth Mutational Profiling in Wastewater
Margaritis Avgeris, Panagiotis G. Adamopoulos, ..., James K. Bashkin
International Journal of Molecular Science… | 2021 Aug 07 | 34445204
Microbiomes in an acidic rock-water cave system.
Katja Burow, Anja Grawunder, ..., Erika Kothe
FEMS Microbiology Letters | 2020 Jun 16 | 31365079
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