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For general laboratory use.
Description:
The enzymes involved in hydrolysis of nucleoside triphosphates are a major focus of biochemical research, targeting the understanding and treatment of cellular processes involved in diseases such as cancer, viral infections, cardiovascular and motional disorders. To elucidate the mechanisms of action of these enzymes, it is often desirable to trap the enzymes in their triphosphate-bound state. Since natural nucleotides are hydrolyzed at rates usually preventing such studies, nucleotide analogs are used that are hydrolyzed at much smaller rates or that are even completely resistant to hydrolysis.The Non-hydrolyzable GTP Test Kit contains a set of 5 typical non-hydrolyzable Guanosine-5'-triphosphate analogs.
The position of the modification within the triphosphate exerts influence on its binding affinity to a particular enzyme and its hydrolysis rate.
This Kit is designed to identify the most suitable non-hydrolyzable GTP derivative for your application.Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Content:
Non-hydrolyzable Guanosine
NucleotideCat. No. Amount GTPαS NU-409S 25 μl (100 mM) GpCpp NU-405S 100 μl (10 mM) GppCp* NU-402-5 5 mg GppNHp* NU-401-10 10 mg GTPγS* NU-412-2 2 mg * Included as a solid for stability reasons. Please dissolve in water and adjust concentration photometrically (extinction coefficient ε252nm = 13.700 M-1cm-1) prior to use to obtain a fresh solution.
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产品组分
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型号
规格 储存温度
非水解GTP检测试剂盒
NK102-01 5pack
-20°C 非水解GTP检测试剂盒
NK102-02 10pack
-20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
Shiba et al. (2002) Hypocretin stimulates [ (35)]GTP gamma S binding in Hcrtr 2-transfected cell lines and in brain homogenate. Biochem. Bioph. Res. Co. 294 (3):615.
Esters et al. (2001) High-resolution crystal structure of S. cerevisiae Ypt51 (Delta C15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis. J. Mol. Biol. 298 (1):111.
Ryder et al. (1999) Nucleotide analog interference mapping. Methods 18 (1):38.
Muller-Reichert et al. (1998) Structural changes at microtubule ends accompanying GTP hydrolysis: information from a slowly hydrolyzable analogue of GTP, guanylyl (alpha,beta) methylenediphosphonate. Proc. Natl. Acad. Sci. USA 95 (7):3661.
Dye et al. (1996) Assembly of microtubules from tubulin bearing the nonhydrolyzable guanosine triphosphate analogue GMPPCP [Guanylyl 5'- (beta,gamma-methylenediphosphonate)]: Variability of growth rates and the hydrolysis of GTP. Biochemistry-US 35 (45):14331.5'-Triphosphate guanosine RNAs recruit GTP-binding proteins to suppress RIG-I/IFN type I signaling.
Magdalena Wolczyk, Jacek Szymanski, ..., Gracjan Michlewski
Molecular cell | 2026 May 08 | 42105765
Supplementary Expression and purification of Escherichia coli RtcB. The rtcB gene was amplified from E. coli strain MG1655 genomic DNA by PCR using the primers: forward, 5′-TAT GCA TGC ACC ATC ATC ATC ACC ATG GTA ATT
pm22320833 | 2025 Sep 28
RNA 5ʹ terminal nucleotide determines the strength of the RIG-I/IFN signaling pathway
Wolczyk Magdalena, Szymanski Jacek, ..., Michlewski Gracjan
bioRxiv | 2023 Dec 22
Development of assays to support identification and characterization of modulators of DExH-box helicase DHX9.
Deepali Gotur, April Case, ..., P Ann Boriack-Sjodin
SLAS discovery : advancing life sciences R… | 2023 Dec 21 | 37625785
GTP binding regulates cellular localization of Parkinson's disease-associated LRRK2
Marian Blanca Ramírez, Antonio Jesús Lara Ordóñez, ..., Sabine Hilfiker
Human molecular genetics | 2017 Jul 15 | 28453723
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