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Background
The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas9 (CRISPR associated system or CRISPR associated protein 9 nuclease) found in bacteria to work as a defense mechanism against foreign DNA has proven to be an invaluable tool to target and modify a genetic sequence in gene editing and genome engineering applications. The system, known as CRISPR/Cas9, allows for sequence-specific cleavage of a targeted genomic locus by delivering the RNA-guided nuclease (Cas9) and appropriate guide RNAs (gRNA) into a cell. In addition, Protospacer Adjacent Motif (PAM) sequence immediately following the specificity sequence is necessary for successful binding of the Cas9 nuclease.Concentration
1 mg/mlDescription
Mouse monoclonal antibody raised against CRISPR/Cas9, clone 7A9, generated with synthesized peptide corresponding to sequence of Streptococcus pyogene (S. pyogenes) CRISPR-associated endonuclease Cas9/Csn1. This Anti-Cas9 mAb can detect CRISPR/Cas9 expression in target cells by WB, IF, IP, or ELISA to confirm and verify whether gRNA and Cas9 vectors are successfully transfected.Specificity
Recognizes both Cas9 and dCas9 (nuclease deficient Cas9)Reactivity
Species IndependentIsotype
IgG1/KappaImmunogen
Recombinant N-terminal fragment of S. pyogenes Cas9 protein expressed in E. coli.Formulation
PBS, 30% GlycerolStorage
Store at 4°C for short term (1-2 weeks). For long term storage, aliquot and then store at −20°C. Avoid multiple freeze/thaw cycles.Purity
Protein G PurifiedAlternative Names
Anti-Cas9, Anti-CRISPR, Anti-CRISPR/Cas9, CRISPR antibody, Cas9 antibody, Cas9 7A9, 7A9-3A3Application
WB (1:200-1:500), IP (2 µg/10^6 cells), IF (1:200-1:500), ELISA (1:1000-1:2000) -
产品组分
内容 型号
规格
保存条件
CRISPR/Cas9单克隆抗体[7A9]
E-A-9000-010
10ug -20°C CRISPR/Cas9单克隆抗体[7A9] E-A-9000-050 50ug -20°C
CRISPR/Cas9单克隆抗体[7A9] E-A-9000-100 100ug -20°C
操作手册
1 1 常温
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文件资源
文件下载 文件内容 资源说明 
E-A-9000-CRISPR/Cas9单克隆抗体[7A9] 操作手册 E-A-9000-CRISPR/Cas9单克隆抗体[7A9]相关单页 宣传单页 -
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
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