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DHFR; DHFRP1; Dihydrofolate reductase; DYR; DYR_HUMAN; EC 1.5.1.3
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Source
Recombinant protein corresponding to aa1-187 from human Dihydrofolate reductase, fused to 6X His-Tag at N-terminal, expressed in E.coli.
Molecular Weight
~25.5kD
Amino Acid Sequence
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥90% (SDS-PAGE)
Form
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Grade
Purified
Swiss Prot
P00374
Molecular Weight
25.5
Shipping Temp
Blue Ice
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产品组分
内容
型号
规格 储存温度
重组人源二氢叶酸还原酶(DHFR)氨基酸1-187
584243-01 20ug
-80°C 重组人源二氢叶酸还原酶(DHFR)氨基酸1-187
584243-02 100ug
-80°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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The E3 ligase RAD18-mediated ubiquitination of henipavirus matrix protein promotes its nuclear-cytoplasmic trafficking and viral egress., PMID:39628457
High-throughput virtual screening of Streptomyces spp. metabolites as antiviral inhibitors against the Nipah virus matrix protein., PMID:38968780
Henipaviruses and lyssaviruses target nucleolar treacle protein and regulate ribosomal RNA synthesis., PMID:36479968
Measles and Nipah virus assembly: Specific lipid binding drives matrix polymerization., PMID:35857835
Paramyxovirus-Like Particles as Protein Delivery Vehicles., PMID:34379508
The Structural Features of Henipavirus Matrix Protein Driving Intracellular Trafficking., PMID:33021467
A Novel Strategy for the Development of Vaccines for SARS-CoV-2 (COVID-19) and Other Viruses Using AI and Viral Shell Disorder., PMID:33006287
Replication of a Nipah Virus Encoding a Nuclear-Retained Matrix Protein., PMID:31665345
Nipah Virus-Like Particle Egress Is Modulated by Cytoskeletal and Vesicular Trafficking Pathways: a Validated Particle Proteomics Analysis., PMID:31551400
Nipah virus induces two inclusion body populations: Identification of novel inclusions at the plasma membrane., PMID:31034506
In silico identification and characterization of common epitope-based peptide vaccine for Nipah and Hendra viruses., PMID:28756915
Nuclear localization and secretion competence are conserved among henipavirus matrix proteins., PMID:28056216
The Matrix Protein of Nipah Virus Targets the E3-Ubiquitin Ligase TRIM6 to Inhibit the IKKε Kinase-Mediated Type-I IFN Antiviral Response., PMID:27622505
Eukaryotic elongation factor 1-beta interacts with the 5' untranslated region of the M gene of Nipah virus to promote mRNA translation., PMID:27236461
Production of the virus-like particles of nipah virus matrix protein in Pichia pastoris as diagnostic reagents., PMID:27088434
Morbillivirus and henipavirus attachment protein cytoplasmic domains differently affect protein expression, fusion support and particle assembly., PMID:26813519
C-Terminal DxD-Containing Sequences within Paramyxovirus Nucleocapsid Proteins Determine Matrix Protein Compatibility and Can Direct Foreign Proteins into Budding Particles., PMID:26792745
Nipah Virus Matrix Protein Influences Fusogenicity and Is Essential for Particle Infectivity and Stability., PMID:26676785
Expression and purification of the matrix protein of Nipah virus in baculovirus insect cell system., PMID:26519022
Evidence for ubiquitin-regulated nuclear and subnuclear trafficking among Paramyxovirinae matrix proteins., PMID:25782006
Matrix proteins of Nipah and Hendra viruses interact with beta subunits of AP-3 complexes., PMID:25210190
Virus particle release from glycosphingolipid-enriched microdomains is essential for dendritic cell-mediated capture and transfer of HIV-1 and henipavirus., PMID:24872578
ANP32B is a nuclear target of henipavirus M proteins., PMID:24823948
Nipah virus entry and egress from polarized epithelial cells., PMID:23283941
Ubiquitin-regulated nuclear-cytoplasmic trafficking of the Nipah virus matrix protein is important for viral budding., PMID:21085610
PIV5 M protein interaction with host protein angiomotin-like 1., PMID:19932912
Production of the matrix protein of Nipah virus in Escherichia coli: virus-like particles and possible application for diagnosis., PMID:19666056
The YPLGVG sequence of the Nipah virus matrix protein is required for budding., PMID:19000317
Mutation of YMYL in the Nipah virus matrix protein abrogates budding and alters subcellular localization., PMID:17005661
Production and characterization of monoclonal antibodies against binary ethylenimine inactivated Nipah virus., PMID:16226320
In silico identification of a putative new paramyxovirus related to the Henipavirus genus., PMID:15527844
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