- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
二氢叶酸还原酶（DHFR）是一种将二氢叶酸还原为四氢叶酸的酶，以NADPH作为电子供体，而NADPH可转化为1-碳转移化学中所用的各类四氢叶酸辅因子。DHFR将二氢叶酸转化为四氢叶酸，后者是嘌呤、胸苷酸和特定氨基酸从头合成所需的甲基穿梭体。尽管功能性DHFR基因定位于5号染色体，但在其他染色体上已发现许多无内含子的加工假基因或类二氢叶酸还原酶基因。DHFR缺乏与巨幼细胞性贫血相关。DHFR敲低在2-羟基油酸的抗癌活性中发挥作用。DHFR基因插入/缺失多态性与女性血清和红细胞叶酸浓度的变化有关。
Description
DHFR Human Recombinant fused with a 20 amino acids His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 207 amino acids (1-187 a.a.) and having a molecular mass of 23.6kDa.
The DHFR is purified by proprietary chromatographic techniques.Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The DHFR solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 2mM DTT and 30% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
Specific activity is >2000 pmol/min/ug is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
-
产品组分
内容
型号
规格 储存温度
重组人源二氢叶酸还原酶(DHFR)
ENZ-443-01 10ug
-20°C 重组人源二氢叶酸还原酶(DHFR)
ENZ-443-02 50ug
-20°C 重组人源二氢叶酸还原酶(DHFR)
ENZ-443-03 1mg
-20°C 操作手册
1 1 常温
-
营销中心
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
-
The E3 ligase RAD18-mediated ubiquitination of henipavirus matrix protein promotes its nuclear-cytoplasmic trafficking and viral egress., PMID:39628457
High-throughput virtual screening of Streptomyces spp. metabolites as antiviral inhibitors against the Nipah virus matrix protein., PMID:38968780
Henipaviruses and lyssaviruses target nucleolar treacle protein and regulate ribosomal RNA synthesis., PMID:36479968
Measles and Nipah virus assembly: Specific lipid binding drives matrix polymerization., PMID:35857835
Paramyxovirus-Like Particles as Protein Delivery Vehicles., PMID:34379508
The Structural Features of Henipavirus Matrix Protein Driving Intracellular Trafficking., PMID:33021467
A Novel Strategy for the Development of Vaccines for SARS-CoV-2 (COVID-19) and Other Viruses Using AI and Viral Shell Disorder., PMID:33006287
Replication of a Nipah Virus Encoding a Nuclear-Retained Matrix Protein., PMID:31665345
Nipah Virus-Like Particle Egress Is Modulated by Cytoskeletal and Vesicular Trafficking Pathways: a Validated Particle Proteomics Analysis., PMID:31551400
Nipah virus induces two inclusion body populations: Identification of novel inclusions at the plasma membrane., PMID:31034506
In silico identification and characterization of common epitope-based peptide vaccine for Nipah and Hendra viruses., PMID:28756915
Nuclear localization and secretion competence are conserved among henipavirus matrix proteins., PMID:28056216
The Matrix Protein of Nipah Virus Targets the E3-Ubiquitin Ligase TRIM6 to Inhibit the IKKε Kinase-Mediated Type-I IFN Antiviral Response., PMID:27622505
Eukaryotic elongation factor 1-beta interacts with the 5' untranslated region of the M gene of Nipah virus to promote mRNA translation., PMID:27236461
Production of the virus-like particles of nipah virus matrix protein in Pichia pastoris as diagnostic reagents., PMID:27088434
Morbillivirus and henipavirus attachment protein cytoplasmic domains differently affect protein expression, fusion support and particle assembly., PMID:26813519
C-Terminal DxD-Containing Sequences within Paramyxovirus Nucleocapsid Proteins Determine Matrix Protein Compatibility and Can Direct Foreign Proteins into Budding Particles., PMID:26792745
Nipah Virus Matrix Protein Influences Fusogenicity and Is Essential for Particle Infectivity and Stability., PMID:26676785
Expression and purification of the matrix protein of Nipah virus in baculovirus insect cell system., PMID:26519022
Evidence for ubiquitin-regulated nuclear and subnuclear trafficking among Paramyxovirinae matrix proteins., PMID:25782006
Matrix proteins of Nipah and Hendra viruses interact with beta subunits of AP-3 complexes., PMID:25210190
Virus particle release from glycosphingolipid-enriched microdomains is essential for dendritic cell-mediated capture and transfer of HIV-1 and henipavirus., PMID:24872578
ANP32B is a nuclear target of henipavirus M proteins., PMID:24823948
Nipah virus entry and egress from polarized epithelial cells., PMID:23283941
Ubiquitin-regulated nuclear-cytoplasmic trafficking of the Nipah virus matrix protein is important for viral budding., PMID:21085610
PIV5 M protein interaction with host protein angiomotin-like 1., PMID:19932912
Production of the matrix protein of Nipah virus in Escherichia coli: virus-like particles and possible application for diagnosis., PMID:19666056
The YPLGVG sequence of the Nipah virus matrix protein is required for budding., PMID:19000317
Mutation of YMYL in the Nipah virus matrix protein abrogates budding and alters subcellular localization., PMID:17005661
Production and characterization of monoclonal antibodies against binary ethylenimine inactivated Nipah virus., PMID:16226320
In silico identification of a putative new paramyxovirus related to the Henipavirus genus., PMID:15527844
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
