冷冻玻璃化启动套装

冷冻玻璃化启动套装，Cryo-EM V-Kit，Vitrification Starter Kit，设计为玻璃化入门套件，旨在助力为可溶性蛋白和膜蛋白筛选最佳的玻璃化条件及冷冻电镜载网。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。

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冷冻玻璃化启动套装

所属分类

分子生物学

目录号

XCEM301

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For research use only.

Description:
The Cryo-EM V-Kit contains 10 different surfactants plus glycerol as cryoprotectant and 4 different types of Quantifoil grids. It is designed as a Vitrification Starter Kit to facilitate the search for the optimum vitrification condition and cryo-EM grid for both soluble and membrane proteins.

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C / ambient temperature

Shelf Life: 12 months

Applications:
Optimization of the vitrification process in cryo-EM sample preparation

Content:

Surfactants and Cryoprotectants	Amount	Conc.	CMC	Class
Fluorinated Octyl Maltoside (FOM)	100 μl	0.41% (w/v)	0.07% (w/v)	non-ionic detergent
Hexadecyl-trimethyl-ammonium Bromide (CTAB)	100 μl	0.34% (w/v)	0.03% (w/v)	cationic detergent
n-Decyl-ß-D-Maltoside (DM)	100 μl	0.87% (w/v)	0.09% (w/v)	non-ionic detergent
n-Decyl-α-D-Maltoside (DαM)	100 μl	0.46% (w/v)	0.08% (w/v)	non-ionic detergent
n-Dodecyl-ß-D-Maltoside (DDM)	100 μl	0.09% (w/v)	0.01% (w/v)	non-ionic detergent
Sodium Deoxycholate	100 μl	1.66% (w/v)	0.17% (w/v)	anionic detergent
Triton X-100	100 μl	0.15% (w/v)	0.01% (w/v)	non-ionic detergent
Tween 20	100 μl	1% (w/v)	0.01% (w/v)	non-ionic detergent
CHAPSO	100 μl	2.5% (w/v)	0.5% (w/v)	zwitterionic detergent
Amphipol A8-35	100 μl	5% (w/v)		anionic surfactant
Glycerol	1 ml	30% (w/v)		cryoprotectant

Quantifoil Holey Carbon Films
20x Quantifoil® R 1.2/1.3 on 200 copper mesh
20x Quantifoil® R 1.2/1.3 with ultrathin (2 nm) continuous layer of carbon on top on 200 copper mesh
20x Quantifoil® R 2/1 on 300 copper mesh
20x Quantifoil® R 2/1 with ultrathin (2 nm) continuous layer of carbon on top on 300 copper mesh

产品组分

内容	型号	规格	储存温度
冷冻玻璃化启动套装	M105-01	1ea	-20°C
冷冻玻璃化启动套装	M105-02	2ea	-20°C
操作手册	1	1	常温

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保存建议厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。
FAQ
Selected References:
[1] Noble et al. (2018) Routine Single Particle CryoEM Sample and Grid Characterization by Tomography. DOI: 10.7554/eLife.34257.
[2] Thonghin et al. (2018) Cryo-electron microscopy of membrane proteins. Methods 147:176.
[3] Drulyte et al. (2018) Approaches to altering particle distributions in cryo-electron microscopy sample preparation. Acta Cryst. D 74:560.
[4] Glaeser et al. (2017) Opinion: hazards faced by macromolecules when confined to thin aqueous films. Biophys Rep 3:1.
[5] Gatsogiannis et al. (2016). Membrane insertion of a Tc toxin in near-atomic detail. Nat. Struct. Mol. Biol. 23:884.
[6] Efremov et al. (2015) Architecture and conformational switch mechanism of the ryanodine receptor. Nature 517:39.