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SpheroTribe 提供了一套简单的工具包,可生成稳定且结构完整的3D细胞结构(球体、类器官),不受细胞类型限制。只需将 SpheroTribe 溶液稀释到你选择的培养基中,即可观察细胞形成大小均一的3D球体,收集后用于后续实验分析。
在您选择的培养基中稀释后,我们的浓缩聚合物基溶液可提高培养基黏度,促进细胞间接触。SpheroTribe 提供了一种简单的方法来生成均一的 3D 细胞结构,可更好地控制其大小和形状,且易于处理和清洗,适用于下游实验。
SpheroTribe 在处理高难度细胞、提高细胞聚集效率、减少样本间差异以及提升体外实验(即迁移、增殖、侵袭或免疫细胞浸润)、免疫染色流程、药物筛选或体内移植实验的一致性方面尤为实用。
除了 SpheroTribe 解决方案外,全套工具包还包含U型底板和宽口吸头,让你开展3D细胞培养实验所需的全部设备一应俱全。
25毫升5倍浓度甲基纤维素溶液
10倍体积U型底96孔板
2盒带大开口的96支200微升移液器吸头(共2盒)
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甲基纤维素培养基(SpheroTribe) TDA-SPK-01 2.5ml 2-8°C 甲基纤维素培养基(SpheroTribe) TDA-SPK-02 25ml 2-8°C 操作手册
1 1 常温
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TDA-SPK-2-25-甲基纤维素培养基(SpheroTribe)
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TDA-SPK-2-25-甲基纤维素培养基(SpheroTribe)相关单页
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保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
FAQ
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Original publication from the developpers :
A 3D spheroid model for glioblastoma, Journal of visualized experiments (JOVE), 2020. Joris Guyon, Laetitia Andrique, Nadège Pujol, Gro Vatne Rosland, Gaelle Recher, Andreas Bikfalvi, Thomas Daubon. (https://doi.org/10.3791/60998)
Other publications using SpheroTribe spheroids:
The rise of 3D spheroids in radiobiology for assessing tumour radioresistance. Acta Oncologica 2026. Charalampopoulou, A., De Luca, F., Magro, G., Matoor, N., Barcellini, A., Butella, G., Ivaldi, G. B., Lillo, S., Manti, L., Mereghetti, A., Tabarelli De Fatis, P., Bottone, M. G., & Facoetti, A. (https://doi.org/10.2340/1651-226X.2026.45079)
Daraxonrasib (RMC-6236) is an effective targeted therapy for RAS-mutant neuroblastoma. BioRXiv, 2026. Ronald D. Hill, Krista M. Dalton, Richard Kurupi, Jamie M. Slaughter, Jane Roberts, Yanli Xing, Victor Kehinde, Kun Zhang, Bin Hu, Vita Kraskauskiene, Madelyn R. Lorenz, Jennifer E. Koblinski, Mikhail G. Dozmorov, Konstantinos V. Floros and Anthony C. Faber. (https://doi.org/10.64898/2026.02.19.706849)
Targeting neoantigens conserved across organs and species overcomes tumor immune escape. BioRXiv 2025. Guillaume Mestrallet, Ross W. Ward, Matthew Brown, Jesse Boumelha, Frederika Rentzeperis, Natalie Vaninov, Miriam Saffern, Ezekiel Olumuyide, Prerna Suri, Sreekumar Balan, Leandra Velazquez, Aparna Ananthanarayanan, Zhihong Chen, Aimee L. Lucas, Miriam Merad, Cansu Cimen Bozkus, Nicolas Vabret, Robert M. Samstein, Nina Bhardwaj (https://doi.org/10.1101/2025.09.25.678519)
Customized nanofibers with lung-targeting and retention properties for treating isolated triple-negative breast cancer pulmonary metastases, ACS Nano 2025. Vanessa Bellat, Adam Glaser, Henry Gong, Aman Gill, Young Jae Lee, Paolo Cifani, Tracy Stokol, Linda Vahdat, and Benedict Law. (https://doi.org/10.1021/acsnano.5c02176)
Dendritic cells type 1 control the formation, maintenance, and function of tertiary lymphoid structures in cancer, BioRXiv, 2024. Raphaël Mattiuz, Jesse Boumelha, Pauline Hamon, Jessica Le Berichel, Abishek Vaidya, Brian Y. Soong, Laszlo Halasz, Emir Radkevich, Hye Mi Kim, Matthew D. Park, Romain Donne, Leanna Troncoso, Darwin D'Souza, Medard Ernest Kaiza, Ian P. MacFawn, Meriem Belabed, Guillaume Mestrallet, Etienne Humblin, Raphaël Merand, Clotilde Hennequin, Giorgio Ioannou, Sinem Ozbey, Igor Figueiredo, Samarth Hegde, Alexander Tepper, Hajer Merarda, Erika Nemeth, Simon Goldstein, Amanda M. Reid, Moataz Noureddine, Alexandra Tabachnikova, Jalal Ahmed, Alexandros D. Polydorides, Nina Bhardwaj, Amaia Lujambio, Zhihong Chen, Edgar Gonzalez Kozlova, Seunghee Kim-Schulze, Joshua D. Brody, Michael Schotsaert, Christine Moussion, Sacha Gnjatic, Catherine Sautès-Fridman, Wolf Herman Fridman, Vladimir Roudko, Brian D. Brown, Thomas U. Marron, Jason G. Cyster, Hélène Salmon, Tullia C. Bruno, Nikhil S. Joshi, Alice O. Kamphorst, Miriam Merad. (https://10.1101/2024.12.27.628014)
Veratridine, a plant-derived alkaloid, suppresses the hyperactive Rictor-mTORC2 pathway: a new targeted therapy for primary and metastatic colorectal cancer, Research Square, 2024. Eikanger MM, Sane S, Schraufnagel KS, Slunecka JL, Potts RA, Freeling J, Sereda G, Rasulev B, Brockstein RL, Emon MAB, Saif MTA, Rezvani K. (https://doi.org/10.21203/rs.3.rs-5199838/v1)
Generation of glioblastoma in mice engrafted with human cytomegalovirus-infected astrocytes, Cancer Gene Therapy, 2024. Joris Guyon, Sandy Haidar Ahmad, Ranim El Baba, Mégane Le Quang, Andreas Bikfalvi, Thomas Daubon and Georges Herbein. (https://doi.org/10.1038/s41417-024-00767-7)
Histological analysis of invasive glioblastoma organoids embedded in a 3D collagen matrix, STAR Protocols, 2023. Joris Guyon and Thomas Daubon. (https://doi.org/10.1016/j.xpro.2023.102521)
Lactate dehydrogenases promote glioblastoma growth and invasion via a metabolic symbiosis, EMBO Molecular Medicine, 2022. Joris Guyon, Ignacio Fernandez-Moncada, Claire M Larrieu, Cyrielle L Bouchez, Antonio C Pagano Zottola, Johanna Galvis, Tiffanie Chouleur, Audrey Burban, Kevin Joseph, Vidhya M Ravi, Heidi Espedal, Gro Vatne Røsland ,Boutaina Daher, Aurélien Barre, Benjamin Dartigues, Slim Karkar , Justine Rudewicz, Irati Romero-Garmendia, Barbara Klink, Konrad Greutzmann, Marie-Alix Derieppe,Thibaut Molinie, Nina Obad, Céline Léon, Giorgio Seano, Hrvoje Miletic, Dieter Henrik Heiland, Giovanni Marsicano, Macha Nikolski, Rolf Bjerkvig, Andreas Bikfalvi & Thomas Daubon. (https://doi.org/10.15252/emmm.202115343)
Coordinated macrophage and T cell interactions mediate response to checkpoint blockade in colorectal cancer, BioRXiv, 2025. Guillaume Mestrallet, Matthew Brown, Natalie Vaninov, Nam Woo Cho, Leandra Velazquez, Aparna Ananthanarayanan, Matthew Spitzer, Nicolas Vabret, Cansu Cimen Bozkus, Robert M Samstein, Nina Bhardwaj. (https://doi.org/10.1101/2025.02.12.637954)
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