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D-2-Hydroxyglutarate Assay Kit (ab211070) provides a convenient method to detect D-2-Hydroxyglutarate (D2HG).
How the assay worksIn the D 2 Hydroxyglutarate assay, D-2-Hydroxyglutarate is oxidized to alpha-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD 450 nm. This absorbance is proportional to the amount of D2HG present in the samples.
D 2 Hydroxyglutarate assay protocol summary- Add samples and standards to wells
- Add reaction mix
- Incubate for 1 hour at 37°C
- Analyze with a microplate readerHow other researchers are using ab211070D 2 Hydroxyglutarate Assay Kit (Colorimetric) has been used in a variety of sample types including:
- Human glioma samples 1
- Primary human astrocytes 2
- Human Diffuse large B cell lymphoma 3
References: 1-Chen X al. 2023, 2-Bredel M et al. 2023, 3-Cai Y et al. 2023Other NotesThis product was previously called K213 Biovision D-2-Hydroxyglutarate (D2HG) Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and alpha-Ketoglutarate. In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of alpha ketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG, D-2-Hydroxyglutarate) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is important for early diagnosis, prognosis and the development of therapeutic strategies against these maladies.
REACH authorisationAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
产品组分
内容 型号
规格 储存温度
D-2-羟基戊二酸(D2HG)定量分析试剂盒(比色法)
ab211070 100次
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Frontiers in immunology 16:1599352 | PubMed 40607414
2025
Wuhan strain of SARS-CoV-2 triggers activation of immune evasion machinery similar to the one operated by cancer cells.
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Maryam Abooali, Inna M Yasinska, Gauri Thapa, Xi Lei, Kelly A S da Costa, Stephanie Schlichtner, Steffen M Berger, Elizaveta Fasler-Kan, Nigel J Temperton, Romina Vuono, Vadim V Sumbayev
Life metabolism 3:loae002 | PubMed 39872214
2025
IDH1 mutation inhibits differentiation of astrocytes and glioma cells with low oxoglutarate dehydrogenase expression by disturbing α-ketoglutarate-related metabolism and epigenetic modification.
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Neuro-oncology advances 6:vdae182 | PubMed 39605316
2024
Targeting the mutation in gliomas by CRISPR/Cas precision base editing.
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Remi Weber, Flavio Vasella, Artsiom Klimko, Manuela Silginer, Martine Lamfers, Marian Christoph Neidert, Luca Regli, Gerald Schwank, Michael Weller
Molecules (Basel, Switzerland) 29: | PubMed 39203017
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Preparation and Preclinical Evaluation of F-Labeled Olutasidenib Derivatives for Non-Invasive Detection of Mutated Isocitrate Dehydrogenase 1 (mIDH1).
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