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DX600 是一种angiotensin-converting enzyme 2 (ACE2)的特异抑制剂，不会与ACE 发生交叉反应。
生物活性
产品描述 DX600 是一种angiotensin-converting enzyme 2 (ACE2)的特异抑制剂，不会与ACE 发生交叉反应。
靶点活性 (IC₅₀) ACE2:
体外活性 DX600 is an ACE2 specific inhibitor that does not cross-react with ACE. Three human cell lines (Calu-3, Caco-2, Huh-7) are evaluated for the cell surface expression of ACE2 by flow cytometry and Western blot. Subsequently, whole cell lysates, cell culture supernatant, and live cells are evaluated in the assay. Calu-3 has elevated levels of ACE2 compared to Caco-2 or Huh-7. Calu-3 also demonstrates elevated ACE2 enzymatic activity in all three sources and can be inhibited by DX600.[1]
体内活性 Mouse ACE2-driven Ang-(1-7) formation from Ang II is blocked by MLN-4760 but not by either linear or cyclic DX600. DX600 only blocks human ACE2 activity.[2]
细胞实验 Cell lines: Three human cell lines (Calu-3, Caco-2, Huh-7)
Concentrations: 10 μM
Incubation Time: 1 h
Method: To measure NAb activity against endogenous ACE2, the recombinant ACE2 is replaced by cellular ACE2. 100 μL of cell lysate at 4 mg/mL or same volume of cell culture supernatant is pre-incubated with either PCs at a final concentration of 10 μg/mL, equal volume control serum, DX600, ACE2 specific inhibitor at final concentration of 10 μM, or equal volume of control buffer (PBS) at room temperature for 1 h.化学信息
分子量 3074.36 分子式 C141H185N35O40S2 CAS No. 478188-26-0 Smiles CC(C)C[C@@H]1NC(=O)C2CCCN2C(=O)[C@H](CO)NC(=O)[C@H](CSSC[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](Cc2c[nH]c3ccccc23)NC(=O)[C@H](Cc2c[nH]c3ccccc23)NC(=O)C2CCCN2C(=O)[C@H](Cc2ccc(O)cc2)NC(=O)[C@H](Cc2ccc(O)cc2)NC(=O)[C@H](CCCNC(N)=N)NC1=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)NCC(=O)NCC(N)=O)NC(=O)[C@H](Cc1cnc[nH]1)NC(=O)[C@H](CO)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(C)=O Sequence Ac-Gly-Asp-Tyr-Ser-His-Cys-Ser-Pro-Leu-Arg-Tyr-Pro-Trp-Lys-Cys-Thr-Tyr-Pro-Asp-Pro-Glu-Gly-Gly-Gly-NH₂ Sequence Short GDYSHCSPLRYPWKCTYPDPEGGG -
产品组分
内容
型号
规格 储存温度
DX600 T35324-01 10mg -20°C DX600 T35324-02 20mg -20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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1.Karen Liao, et al. J Immunol Methods. 2013 Mar 29;389(1-2):52-60.
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